Ontology highlight
ABSTRACT:
SUBMITTER: Korotkova N
PROVIDER: S-EPMC134890 | biostudies-literature | 2002 Mar
REPOSITORIES: biostudies-literature
Korotkova Natalia N Chistoserdova Ludmila L Kuksa Vladimir V Lidstrom Mary E ME
Journal of bacteriology 20020301 6
Most serine cycle methylotrophic bacteria lack isocitrate lyase and convert acetyl coenzyme A (acetyl-CoA) to glyoxylate via a novel pathway thought to involve butyryl-CoA and propionyl-CoA as intermediates. In this study we have used a genome analysis approach followed by mutation to test a number of genes for involvement in this novel pathway. We show that methylmalonyl-CoA mutase, an R-specific crotonase, isobutyryl-CoA dehydrogenase, and a GTPase are involved in glyoxylate regeneration. We a ...[more]