Ontology highlight
ABSTRACT:
SUBMITTER: Mikhailov MV
PROVIDER: S-EPMC1356316 | biostudies-literature | 2005 Dec
REPOSITORIES: biostudies-literature
Mikhailov Michael V MV Campbell Jeff D JD de Wet Heidi H Shimomura Kenju K Zadek Brittany B Collins Richard F RF Sansom Mark S P MS Ford Robert C RC Ashcroft Frances M FM
The EMBO journal 20051124 23
ATP-sensitive potassium (K(ATP)) channels conduct potassium ions across cell membranes and thereby couple cellular energy metabolism to membrane electrical activity. Here, we report the heterologous expression and purification of a functionally active K(ATP) channel complex composed of pore-forming Kir6.2 and regulatory SUR1 subunits, and determination of its structure at 18 A resolution by single-particle electron microscopy. The purified channel shows ATP-ase activity similar to that of ATP-bi ...[more]