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Reverse line blot assay for direct identification of seven Streptococcus agalactiae major surface protein antigen genes.

ABSTRACT: We developed a multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB) to detect the genes encoding members of the family of variable surface-localized proteins of Streptococcus agalactiae (group B streptococcus [GBS]), namely, Bca (Calpha), Rib, Epsilon (Epsilon/Alp1/Alp5), Alp2, Alp3, and Alp4, and the immunoglobulin A binding protein, Bac (Cbeta). We used the assay to identify these genes in a collection of well-characterized GBS isolates and reference strains. The results showed that mPCR/RLB avoids the common problems of cross-reaction and nontypability associated with protein typing using antisera. It is as sensitive as, but more practical than, separate gene-specific PCRs and would be suitable for large molecular epidemiological studies of GBS.

SUBMITTER: Zhao Z

PROVIDER: S-EPMC1356620 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature

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Reverse line blot assay for direct identification of seven Streptococcus agalactiae major surface protein antigen genes.

Zhao Zuotao Z Kong Fanrong F Gilbert Gwendolyn L GL

Clinical and vaccine immunology : CVI 20060101 1

We developed a multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB) to detect the genes encoding members of the family of variable surface-localized proteins of Streptococcus agalactiae (group B streptococcus [GBS]), namely, Bca (Calpha), Rib, Epsilon (Epsilon/Alp1/Alp5), Alp2, Alp3, and Alp4, and the immunoglobulin A binding protein, Bac (Cbeta). We used the assay to identify these genes in a collection of well-characterized GBS isolates and reference strains. The results showed ...[more]

PMID: 16426012

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