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Pregenomic RNA is required for efficient incorporation of pol polyprotein into foamy virus capsids.

ABSTRACT: The foamy virus (FV) Pol polyprotein is translated independently of Gag from a spliced mRNA. This method of expression raises the question of how Pol is associated with the viral particle. Using a transient FV vector transfection system, it is shown that pregenomic RNA is required for efficient virion incorporation of functionally active Pol and that protein-protein interactions of Pol with Gag are not sufficient to complete particle assembly.

SUBMITTER: Heinkelein M

PROVIDER: S-EPMC136505 | biostudies-literature | 2002 Oct

REPOSITORIES: biostudies-literature

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Pregenomic RNA is required for efficient incorporation of pol polyprotein into foamy virus capsids.

Heinkelein Martin M Leurs Cordula C Rammling Matthias M Peters Katrin K Hanenberg Helmut H Rethwilm Axel A

Journal of virology 20021001 19

The foamy virus (FV) Pol polyprotein is translated independently of Gag from a spliced mRNA. This method of expression raises the question of how Pol is associated with the viral particle. Using a transient FV vector transfection system, it is shown that pregenomic RNA is required for efficient virion incorporation of functionally active Pol and that protein-protein interactions of Pol with Gag are not sufficient to complete particle assembly. ...[more]

PMID: 12208988

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RNA and protein requirements for incorporation of the Pol protein into foamy virus particles.

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The human foamy virus pol gene is expressed as a Pro-Pol polyprotein and not as a Gag-Pol fusion protein.

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