Ontology highlight
ABSTRACT:
SUBMITTER: Badgett MR
PROVIDER: S-EPMC136581 | biostudies-literature | 2002 Oct
REPOSITORIES: biostudies-literature
Badgett Marty R MR Auer Alexandra A Carmichael Leland E LE Parrish Colin R CR Bull James J JJ
Journal of virology 20021001 20
The genetic trajectory leading to viral attenuation was studied in a canine parvovirus (CPV) strain grown on dog kidney cells for 115 transfers. Consensus sequences of viral populations at passages 0, 3, 30, 50, 80, and 115 were obtained from PCR products covering 86% of the genome; clones from each of the 80th and 115th passages were also sequenced, covering 69% of the genome. Sixteen changes were fixed in the 115th-passage virus sample. Levels of polymorphism were strikingly different over tim ...[more]