Spectral unmixing of flavin autofluorescence components in cardiac myocytes.
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ABSTRACT:
SUBMITTER: Chorvat D
PROVIDER: S-EPMC1367005 | biostudies-literature | 2005 Dec
REPOSITORIES: biostudies-literature
Publications
Spectral unmixing of flavin autofluorescence components in cardiac myocytes.
Chorvat D D Kirchnerova J J Cagalinec M M Smolka J J Mateasik A A Chorvatova A A
Biophysical journal 20051014 6
We applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain. ...[more]
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