Conjugate-based targeting of recombinant adeno-associated virus type 2 vectors by using avidin-linked ligands.

ABSTRACT: The development of targeted vectors, capable of tissue-specific transduction, remains one of the important aspects of vector modification for gene therapy applications. Recombinant adeno-associated virus type 2 (rAAV-2)-based vectors are nonpathogenic, have relatively low immunogenicity, and are capable of long-term transgene expression. AAV-2 vectors bind primarily to heparan sulfate proteoglycan (HSPG), a receptor that is present in many tissues and cell types. Because of the widespread expression of HSPG on many tissues, targeted transduction in vivo appears to be limited with AAV-2 vectors. Thus, development of strategies to achieve transductional targeting will have a profound benefit in the future application of these vectors. We report here a novel conjugate-based targeting method t

SUBMITTER: Ponnazhagan S 

PROVIDER: S-EPMC136730 | biostudies-literature | 2002 Dec

REPOSITORIES: biostudies-literature