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RNAi: finding the elusive endonuclease.

ABSTRACT: RNA interference involves endonucleolytic cleavage of mRNAs at a site determined by complementary siRNAs. Initial cleavage leads to rapid degradation of the message, resulting in a corresponding reduction in the level of the encoded protein. Despite intensive study, the identity of the endonucleolytic activity (designated slicer) has remained obscure. Now, a combination of structural and biochemical analyses provide compelling evidence that human Argonaute2 (Ago2), a protein already known to be a key player in the RNAi pathway, is in fact the missing endonuclease.

SUBMITTER: Lingel A

PROVIDER: S-EPMC1370652 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature

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RNAi: finding the elusive endonuclease.

Lingel Andreas A Izaurralde Elisa E

RNA (New York, N.Y.) 20041101 11

RNA interference involves endonucleolytic cleavage of mRNAs at a site determined by complementary siRNAs. Initial cleavage leads to rapid degradation of the message, resulting in a corresponding reduction in the level of the encoded protein. Despite intensive study, the identity of the endonucleolytic activity (designated slicer) has remained obscure. Now, a combination of structural and biochemical analyses provide compelling evidence that human Argonaute2 (Ago2), a protein already known to be ...[more]

PMID: 15496518

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