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Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli.

ABSTRACT: The C-terminal domain of the UvrC protein (UvrC CTD) is essential for 5' incision in the prokaryotic nucleotide excision repair process. We have determined the three-dimensional structure of the UvrC CTD using heteronuclear NMR techniques. The structure shows two helix-hairpin-helix (HhH) motifs connected by a small connector helix. The UvrC CTD is shown to mediate structure-specific DNA binding. The domain binds to a single-stranded-double-stranded junction DNA, with a strong specificity towards looped duplex DNA that contains at least six unpaired bases per loop ("bubble DNA"). Using chemical shift perturbation experiments, the DNA-binding surface is mapped to the first hairpin region encompassing the conserved glycine-valine-glycine residues followed by lysine-arginine-arginine, a positively charged surface patch and the second hairpin region consisting of glycine-isoleucine-serine. A model for the protein-DNA complex is proposed that accounts for this specificity.

SUBMITTER: Singh S

PROVIDER: S-EPMC137216 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature

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Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli.

Singh S S Folkers G E GE Bonvin A M J J AM Boelens R R Wechselberger R R Niztayev A A Kaptein R R

The EMBO journal 20021101 22

The C-terminal domain of the UvrC protein (UvrC CTD) is essential for 5' incision in the prokaryotic nucleotide excision repair process. We have determined the three-dimensional structure of the UvrC CTD using heteronuclear NMR techniques. The structure shows two helix-hairpin-helix (HhH) motifs connected by a small connector helix. The UvrC CTD is shown to mediate structure-specific DNA binding. The domain binds to a single-stranded-double-stranded junction DNA, with a strong specificity toward ...[more]

PMID: 12426397

Dataset Information

Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli.

Publications

Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli.

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