Ontology highlight
ABSTRACT:
SUBMITTER: Spotts JM
PROVIDER: S-EPMC137557 | biostudies-literature | 2002 Nov
REPOSITORIES: biostudies-literature
Spotts James M JM Dolmetsch Ricardo E RE Greenberg Michael E ME
Proceedings of the National Academy of Sciences of the United States of America 20021101 23
The ability to make sensitive measurements of protein-protein interaction kinetics in single neurons is critical for understanding the molecular and cellular basis of neuronal function. We have developed a reporter technology based on the differential induction of Escherichia coli TEM-1 beta-lactamase (Bla) enzymatic activity that can function as a sensor of the interaction state of two target proteins within single neurons in vivo. To modulate Bla enzymatic activity, we first split the enzyme i ...[more]