Ontology highlight
ABSTRACT:
SUBMITTER: Gorelik J
PROVIDER: S-EPMC138557 | biostudies-literature | 2002 Dec
REPOSITORIES: biostudies-literature
Gorelik J J Shevchuk A A Ramalho M M Elliott M M Lei C C Higgins C F CF Lab Max J MJ Klenerman D D Krauzewicz N N Korchev Y Y
Proceedings of the National Academy of Sciences of the United States of America 20021204 25
We have developed a method for simultaneous recording of high-resolution topography and cell surface fluorescence in a single scan which we call scanning surface confocal microscopy. The resolution of the system allows imaging of individual fluorescent particles in the nanometer range on fixed or live cells. We used this technique to record the interaction of single virus-like particles with the cell surface and demonstrated that single particles sink into the membrane in invaginations reminisce ...[more]