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Representative appressorium stage cDNA library of Magnaporthe grisea.


ABSTRACT: A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a lambdaTriplEx2 vector by SMART cDNA library containing 2.37x10(6) independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.

SUBMITTER: Lu JP 

PROVIDER: S-EPMC1389628 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature

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Representative appressorium stage cDNA library of Magnaporthe grisea.

Lu Jian-ping JP   Liu Tong-bao TB   Yu Xiao-yun XY   Lin Fu-cheng FC  

Journal of Zhejiang University. Science. B 20050201 2


A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a lambdaTriplEx2 vector by SMART cDNA library containing 2.37x10(6) independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the lat  ...[more]

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