Molecular analysis of microbial community in a groundwater sample polluted by landfill leachate and seawater.

ABSTRACT: Seashore landfill aquifers are environments of special physicochemical conditions (high organic load and high salinity), and microbes in leachate-polluted aquifers play a significant role for intrinsic bioremediation. In order to characterize microbial diversity and look for clues on the relationship between microbial community structure and hydrochemistry, a culture-independent examination of a typical groundwater sample obtained from a seashore landfill was conducted by sequence analysis of 16S rDNA clone library. Two sets of universal 16S rDNA primers were used to amplify DNA extracted from the groundwater so that problems arising from primer efficiency and specificity could be reduced. Of 74 clones randomly selected from the libraries, 30 contained unique sequences whose analysis showe

SUBMITTER: Tian YJ 

PROVIDER: S-EPMC1389716 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature