Isolation of Rickettsia felis in the mosquito cell line C6/36.
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ABSTRACT:
SUBMITTER: Horta MC
PROVIDER: S-EPMC1392945 | biostudies-literature | 2006 Feb
REPOSITORIES: biostudies-literature
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Isolation of Rickettsia felis in the mosquito cell line C6/36.
Horta Maurício C MC Labruna Marcelo B MB Durigon Edison L EL Schumaker Teresinha T S TT
Applied and environmental microbiology 20060201 2
We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene). ...[more]
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