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Alpha-Galactosidase Aga27A, an enzymatic component of the Clostridium josui cellulosome.


ABSTRACT: The Clostridium josui aga27A gene encodes the cellulosomal alpha-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various alpha-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A alpha-galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan as a substrate to small saccharides such as melibiose and raffinose.

SUBMITTER: Jindou S 

PROVIDER: S-EPMC139563 | biostudies-literature | 2002 Jan

REPOSITORIES: biostudies-literature

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alpha-Galactosidase Aga27A, an enzymatic component of the Clostridium josui cellulosome.

Jindou Sadanari S   Karita Shuichi S   Fujino Emi E   Fujino Tsuchiyoshi T   Hayashi Hidenori H   Kimura Tetsuya T   Sakka Kazuo K   Ohmiya Kunio K  

Journal of bacteriology 20020101 2


The Clostridium josui aga27A gene encodes the cellulosomal alpha-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various alpha-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A alpha-galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan a  ...[more]

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