Ontology highlight
ABSTRACT:
SUBMITTER: Alberti P
PROVIDER: S-EPMC140048 | biostudies-literature | 2002 Dec
REPOSITORIES: biostudies-literature
Alberti Patrizia P Arimondo Paola B PB Mergny Jean-Louis JL Garestier Thérèse T Hélène Claude C Sun Jian-Sheng JS
Nucleic acids research 20021201 24
A detailed kinetic study of triple helix formation was performed by surface plasmon resonance. Three systems were investigated involving 15mer pyrimidine oligonucleotides as third strands. Rate constants and activation energies were validated by comparison with thermodynamic values calculated from UV-melting analysis. Replacement of a T.A base pair by a C.G pair at either the 5' or the 3' end of the target sequence allowed us to assess mismatch effects and to delineate the mechanism of triple he ...[more]