RCAS-RNAi: a loss-of-function method for the developing chick retina.
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ABSTRACT:
SUBMITTER: Harpavat S
PROVIDER: S-EPMC1402266 | biostudies-literature | 2006
REPOSITORIES: biostudies-literature
Publications
RCAS-RNAi: a loss-of-function method for the developing chick retina.
Harpavat Sanjiv S Cepko Constance L CL
BMC developmental biology 20060122
<h4>Background</h4>The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function.<h4>Results</h4>We show that avian retroviruses can deliver hairpins mediating RNA interference to the developing chick eye. These viruses 'knock down' specific genes in infected areas of the retina. The knock down persists as the retina matures and can be detected using in situ hybridization. Furthermore, t ...[more]
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