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A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes.

ABSTRACT: Two sPPases (soluble inorganic pyrophosphatases, EC 3.6.1.1) have been isolated from the microalga Chlamydomonas reinhardtii. Both are monomeric proteins of organellar localization, the chloroplastic sPPase I [Cr (Ch. reinhardtii)-sPPase I, 30 kDa] is a major isoform and slightly larger protein than the mitochondrial sPPase II (Cr-sPPase II, 24 kDa). They are members of sPPase family I and are encoded by two different cDNAs, as demonstrated by peptide mass fingerprint analysis. Molecular phylogenetic analyses indicated that Cr-sPPase I is closely related to other eukaryotic sPPases, whereas Cr-sPPase II resembles its prokaryotic counterparts. Chloroplastic sPPase I may have replaced a cyanobacterial ancestor very early during plastid evolution. Cr-sPPase II orthologues are found in members of the green photosynthetic lineage, but not in animals or fungi. These two sPPases from photosynthetic eukaryotes are novel monomeric family I sPPases with different molecular phylogenies and cellular localizations.

SUBMITTER: Gomez-Garcia MR

PROVIDER: S-EPMC1409696 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature

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A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes.

Gómez-García María R MR Losada Manuel M Serrano Aurelio A

The Biochemical journal 20060401 1

Two sPPases (soluble inorganic pyrophosphatases, EC 3.6.1.1) have been isolated from the microalga Chlamydomonas reinhardtii. Both are monomeric proteins of organellar localization, the chloroplastic sPPase I [Cr (Ch. reinhardtii)-sPPase I, 30 kDa] is a major isoform and slightly larger protein than the mitochondrial sPPase II (Cr-sPPase II, 24 kDa). They are members of sPPase family I and are encoded by two different cDNAs, as demonstrated by peptide mass fingerprint analysis. Molecular phyloge ...[more]

PMID: 16313235

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Dataset Information

A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes.

Publications

A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes.

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