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Structure and mechanism of a coreceptor for infection by a pathogenic feline retrovirus.

ABSTRACT: Infection of T lymphocytes by the cytopathic retrovirus feline leukemia virus subgroup T (FeLV-T) requires FeLIX, a cellular coreceptor that is encoded by an endogenous provirus and closely resembles the receptor-binding domain (RBD) of feline leukemia virus subgroup B (FeLV-B). We determined the structure of FeLV-B RBD, which has FeLIX activity, to a 2.5-A resolution by X-ray crystallography. The structure of the receptor-specific subdomain of this glycoprotein differs dramatically from that of Friend murine leukemia virus (Fr-MLV), which binds a different cell surface receptor. Remarkably, we find that Fr-MLV RBD also activates FeLV-T infection of cells expressing the Fr-MLV receptor and that FeLV-B RBD is a competitive inhibitor of infection under these conditions. These studies suggest that FeLV-T infection relies on the following property of mammalian leukemia virus RBDs: the ability to couple interaction with one of a variety of receptors to the activation of a conserved membrane fusion mechanism. A comparison of the FeLV-B and Fr-MLV RBD structures illustrates how receptor-specific regions are linked to conserved elements critical for postbinding events in virus entry.

SUBMITTER: Barnett AL

PROVIDER: S-EPMC141074 | biostudies-literature | 2003 Feb

REPOSITORIES: biostudies-literature

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Structure and mechanism of a coreceptor for infection by a pathogenic feline retrovirus.

Barnett Anna L AL Wensel David L DL Li Weihua W Fass Deborah D Cunningham James M JM

Journal of virology 20030201 4

Infection of T lymphocytes by the cytopathic retrovirus feline leukemia virus subgroup T (FeLV-T) requires FeLIX, a cellular coreceptor that is encoded by an endogenous provirus and closely resembles the receptor-binding domain (RBD) of feline leukemia virus subgroup B (FeLV-B). We determined the structure of FeLV-B RBD, which has FeLIX activity, to a 2.5-A resolution by X-ray crystallography. The structure of the receptor-specific subdomain of this glycoprotein differs dramatically from that of ...[more]

PMID: 12552012

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Structure and mechanism of a coreceptor for infection by a pathogenic feline retrovirus.

Publications

Structure and mechanism of a coreceptor for infection by a pathogenic feline retrovirus.

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