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The chromatoid body of male germ cells: similarity with processing bodies and presence of Dicer and microRNA pathway components.

ABSTRACT: The chromatoid body is a perinuclear, cytoplasmic cloud-like structure in male germ cells whose function has remained elusive. Here we show that the chromatoid body is related to the RNA-processing body of somatic cells. Dicer and components of microRNP complexes (including Ago proteins and microRNAs) are highly concentrated in chromatoid bodies. Furthermore, we show that Dicer interacts with a germ cell-specific chromatoid body component, the RNA helicase MVH (mouse VASA homolog). Thus, chromatoid bodies seem to operate as intracellular nerve centers of the microRNA pathway. Our findings underscore the importance of posttranscriptional gene regulation and of the microRNA pathway in the control of postmeiotic male germ cell differentiation.

SUBMITTER: Kotaja N

PROVIDER: S-EPMC1413789 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature

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The chromatoid body of male germ cells: similarity with processing bodies and presence of Dicer and microRNA pathway components.

Kotaja Noora N Bhattacharyya Suvendra N SN Jaskiewicz Lukasz L Kimmins Sarah S Parvinen Martti M Filipowicz Witold W Sassone-Corsi Paolo P

Proceedings of the National Academy of Sciences of the United States of America 20060213 8

The chromatoid body is a perinuclear, cytoplasmic cloud-like structure in male germ cells whose function has remained elusive. Here we show that the chromatoid body is related to the RNA-processing body of somatic cells. Dicer and components of microRNP complexes (including Ago proteins and microRNAs) are highly concentrated in chromatoid bodies. Furthermore, we show that Dicer interacts with a germ cell-specific chromatoid body component, the RNA helicase MVH (mouse VASA homolog). Thus, chromat ...[more]

PMID: 16477042

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Circadian proteins CLOCK and BMAL1 in the chromatoid body, a RNA processing granule of male germ cells.

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SMG6 localizes to the chromatoid body and shapes the male germ cell transcriptome to drive spermatogenesis.

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The Atlas of Chromatoid Body Components

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-01-24 | GSE54288 | GEO

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