Identification of secreted cysteine proteases from the parasitic nematode Haemonchus contortus detected by biotinylated inhibitors.
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ABSTRACT:
SUBMITTER: Yatsuda AP
PROVIDER: S-EPMC1418636 | biostudies-literature | 2006 Mar
REPOSITORIES: biostudies-literature
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Identification of secreted cysteine proteases from the parasitic nematode Haemonchus contortus detected by biotinylated inhibitors.
Yatsuda Ana P AP Bakker Nicole N Krijgsveld Jeroen J Knox David P DP Heck Albert J R AJ de Vries Erik E
Infection and immunity 20060301 3
Seven cathepsin B-like cysteine proteases (CBLs) were identified from the immunoprotective excretory-secretory products of Haemonchus contortus. Two-dimensional (2-D) zymography and biotinylated inhibitors were employed to localize active CBLs in 2-D protein gels. Mass spectrometry provided the identification of AC-4, HMCP1, HMCP2, and GCP7 as well as three novel CBLs encoded by clustered expressed sequence tags. ...[more]
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