Ontology highlight
ABSTRACT:
SUBMITTER: Suzuki S
PROVIDER: S-EPMC1447452 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Journal of bacteriology 20060501 9
The gene hyuP from Microbacterium liquefaciens AJ 3912 with an added His6 tag was cloned into the expression plasmid pTTQ18 in an Escherichia coli host strain. The transformed E. coli showed transport of radioisotope-labeled 5-substituted hydantoins with apparent K(m) values in the micromolar range. This activity exhibited a pH optimum of 6.6 and was inhibited by dinitrophenol, indicating the requirement of energy for the transport system. 5-Indolyl methyl hydantoin and 5-benzyl hydantoin were t ...[more]