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Virtual histology of transgenic mouse embryos for high-throughput phenotyping.

ABSTRACT: A bold new effort to disrupt every gene in the mouse genome necessitates systematic, interdisciplinary approaches to analyzing patterning defects in the mouse embryo. We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with scan times of 2 h or 12 h, respectively, using mid-gestation E9.5-E12.5 embryos. The datasets generated by this method are immediately amenable to state-of-the-art computational methods of organ patterning analysis. This technique to assess embryo anatomy represents a significant improvement in resolution, time, and expense for the quantitative, three-dimensional analysis of developmental patterning defects attributed to genetically engineered mutations and chemically induced embryotoxicity.

SUBMITTER: Johnson JT

PROVIDER: S-EPMC1449902 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature

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Virtual histology of transgenic mouse embryos for high-throughput phenotyping.

Johnson John T JT Hansen Mark S MS Wu Isabel I Healy Lindsey J LJ Johnson Christopher R CR Jones Greg M GM Capecchi Mario R MR Keller Charles C

PLoS genetics 20060428 4

A bold new effort to disrupt every gene in the mouse genome necessitates systematic, interdisciplinary approaches to analyzing patterning defects in the mouse embryo. We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with sca ...[more]

PMID: 16683035

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Dataset Information

Virtual histology of transgenic mouse embryos for high-throughput phenotyping.

Publications

Virtual histology of transgenic mouse embryos for high-throughput phenotyping.

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