Dataset Information

Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map.

ABSTRACT: The concept of selective (or bin) mapping is used here for the first time, using as an example the Prunus reference map constructed with an almond x peach F2 population. On the basis of this map, a set of six plants that jointly defined 65 possible different genotypes for the codominant markers mapped on it was selected. Sixty-three of these joint genotypes corresponded to a single chromosomal region (a bin) of the Prunus genome, and the two remaining corresponded to two bins each. The 67 bins defined by these six plants had a 7.8-cM average length and a maximum individual length of 24.7 cM. Using a unit of analysis composed of these six plants, their F1 hybrid parent, and one of the parents of the hybrid, we mapped 264 microsatellite (or simple-sequence repeat, SSR) markers from 401 different microsatellite primer pairs. Bin mapping proved to be a fast and economic strategy that could be used for further map saturation, the addition of valuable markers (such as those based on microsatellites or ESTs), and giving a wider scope to, and a more efficient use of, reference mapping populations.

SUBMITTER: Howad W

PROVIDER: S-EPMC1456826 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature

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Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map.

Howad Werner W Yamamoto Toshiya T Dirlewanger Elisabeth E Testolin Raffaele R Cosson Patrick P Cipriani Guido G Monforte Antonio J AJ Georgi Laura L Abbott Albert G AG Arús Pere P

Genetics 20050822 3

The concept of selective (or bin) mapping is used here for the first time, using as an example the Prunus reference map constructed with an almond x peach F2 population. On the basis of this map, a set of six plants that jointly defined 65 possible different genotypes for the codominant markers mapped on it was selected. Sixty-three of these joint genotypes corresponded to a single chromosomal region (a bin) of the Prunus genome, and the two remaining corresponded to two bins each. The 67 bins d ...[more]

PMID: 16118196

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Dataset Information

Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map.

Publications

Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map.

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