Definition of the bacterial N-glycosylation site consensus sequence.
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ABSTRACT:
SUBMITTER: Kowarik M
PROVIDER: S-EPMC1456941 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Publications
Definition of the bacterial N-glycosylation site consensus sequence.
Kowarik Michael M Young N Martin NM Numao Shin S Schulz Benjamin L BL Hug Isabelle I Callewaert Nico N Mills Dominic C DC Watson David C DC Hernandez Marcela M Kelly John F JF Wacker Michael M Aebi Markus M
The EMBO journal 20060413 9
The Campylobacter jejuni pgl locus encodes an N-linked protein glycosylation machinery that can be functionally transferred into Escherichia coli. In this system, we analyzed the elements in the C. jejuni N-glycoprotein AcrA required for accepting an N-glycan. We found that the eukaryotic primary consensus sequence for N-glycosylation is N terminally extended to D/E-Y-N-X-S/T (Y, X not equalP) for recognition by the bacterial oligosaccharyltransferase (OST) PglB. However, not all consensus seque ...[more]
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