Ontology highlight
ABSTRACT:
SUBMITTER: Laptenko O
PROVIDER: S-EPMC1462974 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
The EMBO journal 20060420 10
Gfh1, a transcription factor from Thermus thermophilus, inhibits all catalytic activities of RNA polymerase (RNAP). We characterized the Gfh1 structure, function and possible mechanism of action and regulation. Gfh1 inhibits RNAP by competing with NTPs for coordinating the active site Mg2+ ion. This coordination requires at least two aspartates at the tip of the Gfh1 N-terminal coiled-coil domain (NTD). The overall structure of Gfh1 is similar to that of the Escherichia coli transcript cleavage ...[more]