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Spectral signal-to-noise ratio and resolution assessment of 3D reconstructions.


ABSTRACT: Measuring the quality of three-dimensional (3D) reconstructed biological macromolecules by transmission electron microscopy is still an open problem. In this article, we extend the applicability of the spectral signal-to-noise ratio (SSNR) to the evaluation of 3D volumes reconstructed with any reconstruction algorithm. The basis of the method is to measure the consistency between the data and a corresponding set of reprojections computed for the reconstructed 3D map. The idiosyncrasies of the reconstruction algorithm are taken explicitly into account by performing a noise-only reconstruction. This results in the definition of a 3D SSNR which provides an objective indicator of the quality of the 3D reconstruction. Furthermore, the information to build the SSNR can be used to produce a volumetric SSNR (VSSNR). Our method overcomes the need to divide the data set in two. It also provides a direct measure of the performance of the reconstruction algorithm itself; this latter information is typically not available with the standard resolution methods which are primarily focused on reproducibility alone.

SUBMITTER: Unser M 

PROVIDER: S-EPMC1464087 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature

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Spectral signal-to-noise ratio and resolution assessment of 3D reconstructions.

Unser M M   Sorzano C O S CO   Thévenaz P P   Jonić S S   El-Bez C C   De Carlo S S   Conway J F JF   Trus B L BL  

Journal of structural biology 20050301 3


Measuring the quality of three-dimensional (3D) reconstructed biological macromolecules by transmission electron microscopy is still an open problem. In this article, we extend the applicability of the spectral signal-to-noise ratio (SSNR) to the evaluation of 3D volumes reconstructed with any reconstruction algorithm. The basis of the method is to measure the consistency between the data and a corresponding set of reprojections computed for the reconstructed 3D map. The idiosyncrasies of the re  ...[more]

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