Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli.
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ABSTRACT:
SUBMITTER: Bruant G
PROVIDER: S-EPMC1472324 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
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Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli.
Bruant Guillaume G Maynard Christine C Bekal Sadjia S Gaucher Isabelle I Masson Luke L Brousseau Roland R Harel Josée J
Applied and environmental microbiology 20060501 5
An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance genes was designed and validated using DNA from known reference strains. This microarray was confirmed to be a powerful diagnostic tool for monitoring emerging E. coli pathotypes and antimicrobial resistance, as well as for environmental, epidemiological, and phylogenetic studies including the evaluation of genome plasticity. ...[more]
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