Ontology highlight
ABSTRACT:
SUBMITTER: Bakker H
PROVIDER: S-EPMC1472489 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Bakker Hans H Rouwendal Gerard J A GJ Karnoup Anton S AS Florack Dion E A DE Stoopen Geert M GM Helsper Johannes P F G JP van Ree Ronald R van Die Irma I Bosch Dirk D
Proceedings of the National Academy of Sciences of the United States of America 20060504 20
N-glycosylation of a mAb may have a major impact on its therapeutic merits. Here, we demonstrate that expression of a hybrid enzyme (called xylGalT), consisting of the N-terminal domain of Arabidopsis thaliana xylosyltransferase and the catalytic domain of human beta-1,4-galactosyltransferase I (GalT), in tobacco causes a sharp reduction of N-glycans with potentially immunogenic core-bound xylose (Xyl) and fucose (Fuc) residues as shown by Western blot and MALDI-TOF MS analysis. A radioallergoso ...[more]