Ontology highlight
ABSTRACT:
SUBMITTER: Gut H
PROVIDER: S-EPMC1478166 | biostudies-literature | 2006 Jun
REPOSITORIES: biostudies-literature
Gut Heinz H Pennacchietti Eugenia E John Robert A RA Bossa Francesco F Capitani Guido G De Biase Daniela D Grütter Markus G MG
The EMBO journal 20060504 11
Escherichia coli and other enterobacteria exploit the H+ -consuming reaction catalysed by glutamate decarboxylase to survive the stomach acidity before reaching the intestine. Here we show that chloride, extremely abundant in gastric secretions, is an allosteric activator producing a 10-fold increase in the decarboxylase activity at pH 5.6. Cooperativity and sensitivity to chloride were lost when the N-terminal 14 residues, involved in the formation of two triple-helix bundles, were deleted by m ...[more]