Ontology highlight
ABSTRACT:
SUBMITTER: Rhode BM
PROVIDER: S-EPMC1478171 | biostudies-literature | 2006 Jun
REPOSITORIES: biostudies-literature
Rhode Britta M BM Hartmuth Klaus K Westhof Eric E Lührmann Reinhard R
The EMBO journal 20060511 11
Major structural changes occur in the spliceosome during its catalytic activation, which immediately precedes the splicing of pre-mRNA. Whereas changes in snRNA conformation are well documented at the level of secondary RNA-RNA interactions, little is known about the tertiary structure of this RNA-RNA network, which comprises the spliceosome's catalytic core. Here, we have used the hydroxyl-radical probe Fe-BABE, tethered to the tenth nucleotide (U(+10)) of the 5' end of a pre-mRNA intron, to ma ...[more]