Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection.
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ABSTRACT:
SUBMITTER: Kamachi K
PROVIDER: S-EPMC1479160 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Publications
Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection.
Kamachi Kazunari K Toyoizumi-Ajisaka Hiromi H Toda Kohei K Soeung Sann Chan SC Sarath Svay S Nareth Ya Y Horiuchi Yoshinobu Y Kojima Kazunobu K Takahashi Motohide M Arakawa Yoshichika Y
Journal of clinical microbiology 20060501 5
We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific ...[more]
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