Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing.
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ABSTRACT:
SUBMITTER: Williams LE
PROVIDER: S-EPMC1489313 | biostudies-literature | 2006 Jul
REPOSITORIES: biostudies-literature
Publications
Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing.
Williams Laura E LE Detter Chris C Barry Kerrie K Lapidus Alla A Summers Anne O AO
Applied and environmental microbiology 20060701 7
Sequencing of the large (>50 kb), low-copy-number (<5 per cell) plasmids that mediate horizontal gene transfer has been hindered by the difficulty and expense of isolating DNA from individual plasmids of this class. We report here that a kit method previously devised for purification of bacterial artificial chromosomes (BACs) can be adapted for effective preparation of individual plasmids up to 220 kb from wild gram-negative and gram-positive bacteria. Individual plasmid DNA recovered from less ...[more]
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2012-01-25 | GSE31018 | GEO