CDNA microarray analysis of host-pathogen interactions in a porcine in vitro model for Toxoplasma gondii infection.
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ABSTRACT:
SUBMITTER: Okomo-Adhiambo M
PROVIDER: S-EPMC1489723 | biostudies-literature | 2006 Jul
REPOSITORIES: biostudies-literature
Publications
cDNA microarray analysis of host-pathogen interactions in a porcine in vitro model for Toxoplasma gondii infection.
Okomo-Adhiambo Margaret M Beattie Craig C Rink Anette A
Infection and immunity 20060701 7
Toxoplasma gondii induces the expression of proinflammatory cytokines, reorganizes organelles, scavenges nutrients, and inhibits apoptosis in infected host cells. We used a cDNA microarray of 420 annotated porcine expressed sequence tags to analyze the molecular basis of these changes at eight time points over a 72-hour period in porcine kidney epithelial (PK13) cells infected with T. gondii. A total of 401 genes with Cy3 and Cy5 spot intensities of >/=500 were selected for analysis, of which 26 ...[more]
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