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Discrimination of human pathogenic subspecies of Francisella tularensis by using restriction fragment length polymorphism.

ABSTRACT: We describe the use of two insertion sequence elements (ISFtu1 and ISFtu2) in Francisella tularensis to type strains by restriction fragment length polymorphism (RFLP). The RFLP profiles of 17 epidemiologically unrelated isolates were determined and compared. Our results showed that RFLP profiles can be used to assign F. tularensis strains into five main groups corresponding to strains of F. tularensis subsp. tularensis, F. tularensis strain ATCC 6223, strains of F. tularensis subsp. holarctica, strains of F. tularensis subsp. holarctica from Japan, and F. tularensis subsp. mediaasiatica. The results confirm the genetic identities of these subspecies and also support the suggestion that strains of F. tularensis subsp. holarctica from Japan should be considered members of a separate biovar. These findings should support future studies to determine the genetic differences between strains of F. tularensis at the whole-genome level.

SUBMITTER: Thomas R 

PROVIDER: S-EPMC149632 | biostudies-literature | 2003 Jan

REPOSITORIES: biostudies-literature

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Discrimination of human pathogenic subspecies of Francisella tularensis by using restriction fragment length polymorphism.

Thomas Rebecca R   Johansson Anders A   Neeson Brendan B   Isherwood Karen K   Sjöstedt Anders A   Ellis Jill J   Titball Richard W RW  

Journal of clinical microbiology 20030101 1

We describe the use of two insertion sequence elements (ISFtu1 and ISFtu2) in Francisella tularensis to type strains by restriction fragment length polymorphism (RFLP). The RFLP profiles of 17 epidemiologically unrelated isolates were determined and compared. Our results showed that RFLP profiles can be used to assign F. tularensis strains into five main groups corresponding to strains of F. tularensis subsp. tularensis, F. tularensis strain ATCC 6223, strains of F. tularensis subsp. holarctica,  ...[more]

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