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Rapid identification of the genus fonsecaea by PCR with specific oligonucleotide primers.

ABSTRACT: An oligonucleotide primer set based on internal transcribed spacer regions of ribosomal DNA for PCR which gives the amplicon for only the DNA from Fonsecaea species was designed. This set yielded an amplicon with 333 bp for all strains of Fonsecaea pedrosoi and Fonsecaea compacta examined but no amplicons for related dematiaceous fungi and pathogenic yeasts. PCR using this primer set was considered to be a useful method for the rapid identification of the genus FONSECAEA:

SUBMITTER: Abliz P

PROVIDER: S-EPMC149654 | biostudies-literature | 2003 Feb

REPOSITORIES: biostudies-literature

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Rapid identification of the genus fonsecaea by PCR with specific oligonucleotide primers.

Abliz Paride P Fukushima Kazutaka K Takizawa Kayoko K Nieda Norikazu N Miyaji Makoto M Nishimura Kazuko K

Journal of clinical microbiology 20030201 2

An oligonucleotide primer set based on internal transcribed spacer regions of ribosomal DNA for PCR which gives the amplicon for only the DNA from Fonsecaea species was designed. This set yielded an amplicon with 333 bp for all strains of Fonsecaea pedrosoi and Fonsecaea compacta examined but no amplicons for related dematiaceous fungi and pathogenic yeasts. PCR using this primer set was considered to be a useful method for the rapid identification of the genus FONSECAEA: ...[more]

PMID: 12574304

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