Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR.
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ABSTRACT:
SUBMITTER: Heller LC
PROVIDER: S-EPMC150052 | biostudies-literature | 2003 Mar
REPOSITORIES: biostudies-literature
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Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR.
Heller Loree C LC Davis Carisa R CR Peak K Kealy KK Wingfield David D Cannons Andrew C AC Amuso Philip T PT Cattani Jacqueline J
Applied and environmental microbiology 20030301 3
In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. ...[more]
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