A statistical method for predicting splice variants between two groups of samples using GeneChip expression array data.

ABSTRACT:

Background

Alternative splicing of pre-messenger RNA results in RNA variants with combinations of selected exons. It is one of the essential biological functions and regulatory components in higher eukaryotic cells. Some of these variants are detectable with the Affymetrix GeneChip that uses multiple oligonucleotide probes (i.e. probe set), since the target sequences for the multiple probes are adjacent within each gene. Hybridization intensity from a probe correlates with abundance of the corresponding transcript. Although the multiple-probe feature in the current GeneChip was designed to assess expression values of individual genes, it also measures transcriptional abundance for a sub-region of a gene sequence. This additional capacity motivated us to develop a method to predict

SUBMITTER: Fan W 

PROVIDER: S-EPMC1502129 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature