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Novel sample preparation method for safe and rapid detection of Bacillus anthracis spores in environmental powders and nasal swabs.


ABSTRACT: Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores.

SUBMITTER: Luna VA 

PROVIDER: S-EPMC150283 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature

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Novel sample preparation method for safe and rapid detection of Bacillus anthracis spores in environmental powders and nasal swabs.

Luna Vicki A VA   King Debra D   Davis Carisa C   Rycerz Tony T   Ewert Matthew M   Cannons Andrew A   Amuso Philip P   Cattani Jacqueline J  

Journal of clinical microbiology 20030301 3


Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores. ...[more]

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