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Identification of Histoplasma capsulatum from culture extracts by real-time PCR.

ABSTRACT: We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.

SUBMITTER: Martagon-Villamil J 

PROVIDER: S-EPMC150317 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature

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Identification of Histoplasma capsulatum from culture extracts by real-time PCR.

Martagon-Villamil Jose J   Shrestha Nabin N   Sholtis Mary M   Isada Carlos M CM   Hall Gerri S GS   Bryne Terry T   Lodge Barbara A BA   Reller L Barth LB   Procop Gary W GW  

Journal of clinical microbiology 20030301 3

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism. ...[more]

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