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Transcriptional analysis of the B cell germinal center reaction.

ABSTRACT: The germinal center (GC) reaction is crucial for T cell-dependent immune responses and is targeted by B cell lymphomagenesis. Here we analyzed the transcriptional changes that occur in B cells during GC transit (naive B cells --> centroblasts --> centrocytes --> memory B cells) by gene expression profiling. Naive B cells, characterized by the expression of cell cycle-inhibitory and antiapoptotic genes, become centroblasts by inducing an atypical proliferation program lacking c-Myc expression, switching to a proapoptotic program, and down-regulating cytokine, chemokine, and adhesion receptors. The transition from GC to memory cells is characterized by a return to a phenotype similar to that of naive cells except for an apoptotic program primed for both death and survival and for changes in the expression of cell surface receptors including IL-2 receptor beta. These results provide insights into the dynamics of the GC reaction and represent the basis for the analysis of B cell malignancies.

SUBMITTER: Klein U 

PROVIDER: S-EPMC151393 | biostudies-literature | 2003 Mar

REPOSITORIES: biostudies-literature

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Transcriptional analysis of the B cell germinal center reaction.

Klein Ulf U   Tu Yuhai Y   Stolovitzky Gustavo A GA   Keller Jeffrey L JL   Haddad Joseph J   Miljkovic Vladan V   Cattoretti Giorgio G   Califano Andrea A   Dalla-Favera Riccardo R  

Proceedings of the National Academy of Sciences of the United States of America 20030225 5

The germinal center (GC) reaction is crucial for T cell-dependent immune responses and is targeted by B cell lymphomagenesis. Here we analyzed the transcriptional changes that occur in B cells during GC transit (naive B cells --> centroblasts --> centrocytes --> memory B cells) by gene expression profiling. Naive B cells, characterized by the expression of cell cycle-inhibitory and antiapoptotic genes, become centroblasts by inducing an atypical proliferation program lacking c-Myc expression, sw  ...[more]