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Protein splicing of the Deinococcus radiodurans strain R1 Snf2 intein.

ABSTRACT: Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein that splices by the Ala1 protein splicing pathway, with splicing dependent on adjacent residues.

SUBMITTER: Southworth MW

PROVIDER: S-EPMC151952 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature

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Protein splicing of the Deinococcus radiodurans strain R1 Snf2 intein.

Southworth Maurice W MW Perler Francine B FB

Journal of bacteriology 20021101 22

Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein that splices by the Ala1 protein splicing pathway, with splicing dependent on adjacent residues. ...[more]

PMID: 12399510

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