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A genomewide screen for components of the RNAi pathway in Drosophila cultured cells.

ABSTRACT: Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster. The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2, Tis11, Hsc70-3, Hsc70-4, and hdc) and two annotated genes (CG17265 and CG10883).

SUBMITTER: Dorner S

PROVIDER: S-EPMC1525048 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature

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A genomewide screen for components of the RNAi pathway in Drosophila cultured cells.

Dorner Silke S Lum Lawrence L Kim Michelle M Paro Renato R Beachy Philip A PA Green Rachel R

Proceedings of the National Academy of Sciences of the United States of America 20060801 32

Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster. The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2, Tis11, Hsc70-3, Hsc70-4, and hdc) and two annotated genes (CG17265 and CG1 ...[more]

PMID: 16882716

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