Ontology highlight
ABSTRACT:
SUBMITTER: Hunter CL
PROVIDER: S-EPMC152976 | biostudies-literature | 2003 Apr
REPOSITORIES: biostudies-literature
Hunter Christie L CL Maurus Robert R Mauk Marcia R MR Lee Hung H Raven Emma L EL Tong Harry H Nguyen Nham N Smith Michael M Brayer Gary D GD Mauk A Grant AG
Proceedings of the National Academy of Sciences of the United States of America 20030318 7
A binding site for metal ions has been created on the surface of horse heart myoglobin (Mb) near the heme 6-propionate group by replacing K45 and K63 with glutamyl residues. One-dimensional (1)H NMR spectroscopy indicates that Mn(2+) binds in the vicinity of the heme 6-propionate as anticipated, and potentiometric titrations establish that the affinity of the new site for Mn(2+) is 1.28(4) x 10(4) M(-1) (pH 6.96, ionic strength I = 17.2 microM, 25 degrees C). In addition, these substitutions low ...[more]