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FhuA of Actinobacillus pleuropneumoniae encodes a ferrichrome receptor but is not regulated by iron.

ABSTRACT: The swine pathogen Actinobacillus pleuropneumoniae possesses a 75-kDa outer membrane protein (OMP), FhuA, the receptor for ferrichrome, a hydroxamate-type siderophore. Polyclonal serum to FhuA reacted with OMP preparations from 12 serotypes of A. pleuropneumoniae under conditions of iron repletion and restriction. Reverse transcription-PCR confirmed that A. pleuropneumoniae fhuA expression is not upregulated in response to low iron levels. An A. pleuropneumoniae fhuA deletion mutant was generated and showed abolishment of ferrichrome uptake.

SUBMITTER: Mikael LG

PROVIDER: S-EPMC153255 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature

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fhuA of Actinobacillus pleuropneumoniae encodes a ferrichrome receptor but is not regulated by iron.

Mikael Leonie G LG Srikumar Ramakrishnan R Coulton James W JW Jacques Mario M

Infection and immunity 20030501 5

The swine pathogen Actinobacillus pleuropneumoniae possesses a 75-kDa outer membrane protein (OMP), FhuA, the receptor for ferrichrome, a hydroxamate-type siderophore. Polyclonal serum to FhuA reacted with OMP preparations from 12 serotypes of A. pleuropneumoniae under conditions of iron repletion and restriction. Reverse transcription-PCR confirmed that A. pleuropneumoniae fhuA expression is not upregulated in response to low iron levels. An A. pleuropneumoniae fhuA deletion mutant was generate ...[more]

PMID: 12704168

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