Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars Typhi and Paratyphi A.
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ABSTRACT:
SUBMITTER: Hirose K
PROVIDER: S-EPMC153373 | biostudies-literature | 2002 Feb
REPOSITORIES: biostudies-literature
Publications
Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars Typhi and Paratyphi A.
Hirose Kenji K Itoh Ken-Ichiro K Nakajima Hiroshi H Kurazono Takayuki T Yamaguchi Masanori M Moriya Kazuo K Ezaki Takayuki T Kawamura Yoshiaki Y Tamura Kazumichi K Watanabe Haruo H
Journal of clinical microbiology 20020201 2
The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt (rfbS), fliC-d, fliC-a, and viaB, were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay. ...[more]
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