Rapid real-time PCR for determination of penicillin susceptibility in pneumococcal meningitis, including culture-negative cases.
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ABSTRACT:
SUBMITTER: Kearns AM
PROVIDER: S-EPMC153399 | biostudies-literature | 2002 Feb
REPOSITORIES: biostudies-literature
Publications
Rapid real-time PCR for determination of penicillin susceptibility in pneumococcal meningitis, including culture-negative cases.
Kearns Angela M AM Graham Clive C Burdess Doreen D Heatherington Jane J Freeman Roger R
Journal of clinical microbiology 20020201 2
A novel real-time PCR-hybridization assay was developed for the rapid (<1 h) detection of penicillin susceptibility in Streptococcus pneumoniae. When applied to 24 pneumococcal DNA-positive cerebrospinal fluid extracts, penicillin-sensitive S. pneumoniae was detected in all instances. Real-time PCR proved more sensitive than culture, microscopy, or antigen detection and provided susceptibility data even in culture-negative cases. ...[more]
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