Development of a dinoflagellate-oriented PCR primer set leads to detection of picoplanktonic dinoflagellates from Long Island Sound.
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ABSTRACT:
SUBMITTER: Lin S
PROVIDER: S-EPMC1538694 | biostudies-literature | 2006 Aug
REPOSITORIES: biostudies-literature
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Development of a dinoflagellate-oriented PCR primer set leads to detection of picoplanktonic dinoflagellates from Long Island Sound.
Lin Senjie S Zhang Huan H Hou Yubo Y Miranda Lilibeth L Bhattacharya Debashish D
Applied and environmental microbiology 20060801 8
We developed dinoflagellate-specific 18S rRNA gene primers. PCR amplification using these oligonucleotides for a picoplanktonic DNA sample from Long Island Sound yielded 24 clones, and all but one of these clones were dinoflagellates primarily belonging to undescribed and Amoebophrya-like lineages. These results highlight the need for a systematic investigation of picodinoflagellate diversity in both coastal and oceanic ecosystems. ...[more]
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