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Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef.

ABSTRACT: Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8 degrees C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche.

SUBMITTER: Fontana C

PROVIDER: S-EPMC1538697 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature

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Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef.

Fontana Cecilia C Cocconcelli Pier Sandro PS Vignolo Graciela G

Applied and environmental microbiology 20060801 8

Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8 degrees C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversit ...[more]

PMID: 16885317

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