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ABSTRACT:
SUBMITTER: Lockhart RJ
PROVIDER: S-EPMC1538735 | biostudies-literature | 2006 Aug
REPOSITORIES: biostudies-literature
Lockhart Robert J RJ Van Dyke Michele I MI Beadle Ian R IR Humphreys Paul P McCarthy Alan J AJ
Applied and environmental microbiology 20060801 8
Oligonucleotide primers were designed for the 18S rRNA genes of members of the Neocallimastigales and used in a nested PCR protocol to amplify 787-bp fragments of DNA from landfill site samples. The specificities of the primers were confirmed by phylogenetic analysis of the environmental clone sequences, and this method can therefore now be used to investigate the ecology of the obligately anaerobic fungi. To our knowledge, this is the first demonstration of the occurrence of members of the Neoc ...[more]